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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
07/07/2021 |
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Actualizado : |
27/06/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MANISSE, N.; CABRERA, A.; HERNÁN, J.; COSSE, M.; GIANNITTI, F.; FRANCIA, M.E.; GONZÁLEZ, T.; IRIARTE, A.; RIET-CORREA, F.; ROBELLO, C.; GONZÁLEZ, S. |
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Afiliación : |
NATALIA MANISSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; JUAN HERNÁN, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; MARIANA COSSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA E. FRANCIA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; TELMA GONZÁLEZ, Departamento de Parasitología y Micología, Facultad de Medicina, Instituto de Higiene, Universidad de La República, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; ANDRÉS IRIARTE, Laboratorio de Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Universidad de La República Facultad de Medicina, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Programa de Pós-graduação em Ciência Animal nos Trópicos, Universidade Federal da Bahia, Salvador, Bahia, Brazil.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; SUSANA GONZÁLEZ, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay. |
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Título : |
Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Molecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w |
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DOI : |
10.1007/s11033-021-06492-w |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de
Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026). |
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Contenido : |
Abstract:
Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife. |
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Palabras claves : |
Hammondia spp; NEOSPOROSIS; Neotropical canids; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Real-time PCR; TOXOPLASMOSIS. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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Marc : |
LEADER 02795naa a2200349 a 4500
001 1062284
005 2022-06-27
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-021-06492-w$2DOI
100 1 $aMANISSE, N.
245 $aMolecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes$ba contribution to their molecular epidemiology.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026).
520 $aAbstract: Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife.
653 $aHammondia spp
653 $aNEOSPOROSIS
653 $aNeotropical canids
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aReal-time PCR
653 $aTOXOPLASMOSIS
700 1 $aCABRERA, A.
700 1 $aHERNÁN, J.
700 1 $aCOSSE, M.
700 1 $aGIANNITTI, F.
700 1 $aFRANCIA, M.E.
700 1 $aGONZÁLEZ, T.
700 1 $aIRIARTE, A.
700 1 $aRIET-CORREA, F.
700 1 $aROBELLO, C.
700 1 $aGONZÁLEZ, S.
773 $tMolecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
06/08/2019 |
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Actualizado : |
06/08/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MIRABALLES, C.; ARÁOZ, V.; RIET-CORREA, F. |
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Afiliación : |
MÓNICA CECILIA MIRABALLES FERRER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Rhipicephalus microplus, babesiosis and anaplasmosis in Uruguay: current situation and control or elimination programs on farms. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Experimental and Applied Acarology, 2019. |
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DOI : |
10.1007/s10493-019-00405-0 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 16 May 2019 / Accepted: 23 July 2019; Published online: 27july 2019. |
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Contenido : |
Abstract: Rhipicephalus microplus causes high economic losses for Uruguay; the country is divided into a R. microplus-free area and an endemic area. The aim of this research was to evaluate the R. microplus and tick fever situation in Uruguay and the feasibility of implementing R. microplus control or elimination programs to reduce the number of infested farms. A representative survey was performed to determine the number of infested farms, the number of farms with outbreaks of tick fever and the control and preventive measures being used. Control or elimination programs using generational treatment were implemented on 30 farms. Previously, a situation diagnosis was performed by evaluating the background of each farm, identifying acaricide resistance, serologically evaluating the enzootic stability of tick fever, and analyzing the risk of R. microplus introduction to farms. According to the survey, R. microplus was present on 9544 (36%) farms, and 3436 (36%) of these farms experienced outbreaks of tick fever. Only 323 (9.4%) farmers used the tick fever vaccine. Of the 30 farms for which control or elimination programs were carried out, 18 reduced the number of treatments applied per year and six achieved the elimination of the cattle tick. It is concluded that by administering elimination or control programs, it is possible to reduce the number of infested farms and the number of treatments to maintain control. New surveys should be performed to measure the degree of technology adoption by farmers and to
analyze the advances in tick control or elimination programs on farms. MenosAbstract: Rhipicephalus microplus causes high economic losses for Uruguay; the country is divided into a R. microplus-free area and an endemic area. The aim of this research was to evaluate the R. microplus and tick fever situation in Uruguay and the feasibility of implementing R. microplus control or elimination programs to reduce the number of infested farms. A representative survey was performed to determine the number of infested farms, the number of farms with outbreaks of tick fever and the control and preventive measures being used. Control or elimination programs using generational treatment were implemented on 30 farms. Previously, a situation diagnosis was performed by evaluating the background of each farm, identifying acaricide resistance, serologically evaluating the enzootic stability of tick fever, and analyzing the risk of R. microplus introduction to farms. According to the survey, R. microplus was present on 9544 (36%) farms, and 3436 (36%) of these farms experienced outbreaks of tick fever. Only 323 (9.4%) farmers used the tick fever vaccine. Of the 30 farms for which control or elimination programs were carried out, 18 reduced the number of treatments applied per year and six achieved the elimination of the cattle tick. It is concluded that by administering elimination or control programs, it is possible to reduce the number of infested farms and the number of treatments to maintain control. New surveys should be performed to measure the degree of technol... Presentar Todo |
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Palabras claves : |
BOVINE TICK FEVER; CONTROL; ELIMINATION; RHIPICEPHALUS MICROPLUS; URUGUAY. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02386naa a2200229 a 4500
001 1059978
005 2019-08-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s10493-019-00405-0$2DOI
100 1 $aMIRABALLES, C.
245 $aRhipicephalus microplus, babesiosis and anaplasmosis in Uruguay$bcurrent situation and control or elimination programs on farms.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 16 May 2019 / Accepted: 23 July 2019; Published online: 27july 2019.
520 $aAbstract: Rhipicephalus microplus causes high economic losses for Uruguay; the country is divided into a R. microplus-free area and an endemic area. The aim of this research was to evaluate the R. microplus and tick fever situation in Uruguay and the feasibility of implementing R. microplus control or elimination programs to reduce the number of infested farms. A representative survey was performed to determine the number of infested farms, the number of farms with outbreaks of tick fever and the control and preventive measures being used. Control or elimination programs using generational treatment were implemented on 30 farms. Previously, a situation diagnosis was performed by evaluating the background of each farm, identifying acaricide resistance, serologically evaluating the enzootic stability of tick fever, and analyzing the risk of R. microplus introduction to farms. According to the survey, R. microplus was present on 9544 (36%) farms, and 3436 (36%) of these farms experienced outbreaks of tick fever. Only 323 (9.4%) farmers used the tick fever vaccine. Of the 30 farms for which control or elimination programs were carried out, 18 reduced the number of treatments applied per year and six achieved the elimination of the cattle tick. It is concluded that by administering elimination or control programs, it is possible to reduce the number of infested farms and the number of treatments to maintain control. New surveys should be performed to measure the degree of technology adoption by farmers and to analyze the advances in tick control or elimination programs on farms.
653 $aBOVINE TICK FEVER
653 $aCONTROL
653 $aELIMINATION
653 $aRHIPICEPHALUS MICROPLUS
653 $aURUGUAY
700 1 $aARÁOZ, V.
700 1 $aRIET-CORREA, F.
773 $tExperimental and Applied Acarology, 2019.
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